
Reagecon’s turbidity standards for ratio and non-ratio instruments are composed of suspended polymer microspheres.
These turbidity standards remove the handling, stability and accuracy problems associated with traditional Formazin turbidity
standards; (for detailed comparison, see Table 1).
Table 1: Comparison of Reagecon Polymer Microsphere & Formazin Turbidity Standards
Feature | Reagecon Polymer Microspheres | Formazin |
Toxicity |
Non-toxic. No special handling or |
Very toxic, contains a known carcinogen. |
Particle shape & size |
Well defined spherical shape. Mean |
Irregular shape and distribution. |
Shelf life |
Does not deteriorate or settle out. |
Flocculates and deteriorates. |
Particle suspension |
Particles stay in suspension. Mixing is |
Particles settle quickly, |
Traceability |
Certified traceable to NIST Reference |
Non traceable |
Precision (batch to batch) | Mean of SD’s 0±0.00 | Mean of SD’s 0.9±0.2 |
Inter-instrument reproducibility |
0.5 ±0.0 | 0.8±0.2 |
Stability | 0.1 – 4000 NTU (1 year) | 4000 NTU (3 months). Need for dilutions to be prepared daily or weekly |
Accuracy |
Highly accurate for Reagecon Polymer |
±10% (4000 NTUs) up to ±30% for |
Turbidity Measurement
Accurate and precise laboratory or online analytical measurement can be influenced by the following 6 key parameters:
• Measuring Instrument • Measuring Accessories • The Sample
• The Operator • Standards and Reference Material • Measuring Environment
The technical validation, comparability, quality control/assurance, proficiency testing and traceability of any analysis require
significant attention to detail of all these parameters. Turbidity measurement is no different in this respect.
The Standard / Reference Material
The nephelometric turbidity meter is designed to be routinely standardised with a known light scattering standard. As with
all analytical standards or reference materials, a turbidity standard should fulfil the following criteria:
• Provide traceability.
• Demonstrate the accuracy of results.
• Calibrate the equipment and methodology.
• Monitor the user performance.
• Validate the test.
• Facilitate comparability i.e. to ensure that when the correct procedures have been followed the same analysis of the
same materials will produce results that agree with each other whenever they are performed.
Standards and Reference materials should be produced and characterised in a technically competent manner, should be
homogenous, stable, certified and have available a known uncertainty of measurement. Presently, there are only two types
of standards recognised and approved by the USEPA, Standard Methods, ASTM and other regulatory agencies, these are
formazin or formazin derived standards and suspended polymer microspheres.
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